Librarians from 1485 to 1797
- 1485: first "Bibliothecarius Sancti Marci" Marco Barbarigo (later Doge)
- 1485-1486: Agostino Barbarigo, fratello di Marco (later Doge)
- 1486-1506: Marc'Antonio Coccia di Vicovaro known as il Sabellico
- 1515-1529: Andrea Navagero
- 1530-1543: Pietro Bembo
- 1543-1547: Benedetto Ramberti
- 1547-1558: the library was directed by Andrea de’ Franceschi until 1551, then by the Scotsman John Dempster
- 1558-1575: Bernardino Loredan (first Librarian of the Library of St Mark)
- 1575-1582: Alvise Gradenigo
- 158?-1586: Alvise Pesaro
- 1588-1601: Benedetto Zorzi
- 1601-1602: Niccolò Morosini
- 1611-1623: Giovanni Querini
- 1623-1636: Girolamo Soranzo
- 1636-1648: Giovanni Nani
- 1650-1651: Alvise Contarini
- 1659-1678: Battista Nani
- 1679-1694: Silvestro Valier (later Doge)
- 1694-1709: Francesco Corner
- 1709-1735: Girolamo Venier
- 1736-1742: Lorenzo Tiepolo
- 1742-1762: Marco Foscarini (later Doge)
- 1762-1763: Alvise IV Mocenigo (later Doge)
- 1764-1775: Girolamo Grimani
- 1775-1778: Girolamo Ascanio Giustinian
(from 1775 the office was fixed at a three-year term) - 1778-1780: Girolamo Grimani
- 1781-1782: Girolamo Ascanio Giustinian
- 1784-1786: Alvise II Piero Contarini
- 1786-1789: Francesco Pesaro
- 1789-1791: Girolamo Ascanio Giustinian
- 1791-1794: Zaccaria Vallaresso
- 1794-1797: Francesco Pesaro
Custodians from 1485 to 1797
- 1626-1633: Giovanni Sozomeno
- 1633-1658: Santo Damiani
- 1659-1664: Giovanni Matteo Bustronio
- 1664-1669: Philarás Leonardo
- 1669-1680: Alvise-Ambrogio Gradenigo
- 1681-1702: Gualtiero Leith
- 1702-1737: Marcantonio Maderò
- 1737-1778: Anton Maria Zanetti
- 1778-1797: Jacopo Morelli
Directors of the Biblioteca Nazionale Marciana from 1797 to the present day
- Jacopo Morelli: from June 1797 to 1819
- Pietro Bettio: from 1819 to 1846
- Giuseppe Valentinelli: from 1846 to 1874
- Giovanni Veludo: from 1874 to 1884
- Carlo Castellani: from 1884 to 1897
- Salomone Morpurgo: from 1898 to 1905
- Carlo Frati: from December 1905 to July 1913
- Giulio Coggiola: from 1913 to September 1919
- Ester Pastorello (acting): from September 1919 to November 1920
- Luigi Ferrari: from December 1920 to July 1948
- Pietro Zorzanello: from 1948 to 1951
- Tullia Gasparrini Leporace: from 1951 to 1969
- Giorgio Emanuele Ferrari: from 1969 to 1973
- Eugenia Govi: from 1973 to 1976
- Gian Albino Ravalli Modoni: from 1976 to 1989
- Marino Zorzi: from 1989 to 30 November 2007
- Roberto Di Carlo (acting): from December 2007 to April 2008
- Maria Letizia Sebastiani: from May 2008 to February 2012
- Maurizio Messina: February 2012 – 31 July 2018
- Stefano Campagnolo: 4 September 2018 – 15 May 2024
- Stefano Trovato: 16 May 2024 –